|
KMID : 0380620210530040391
|
|
Korean Journal of Food Science and Technology
2021 Volume.53 No. 4 p.391 ~ p.398
|
|
|
Validation and measurement uncertainty of HPLC method for simultaneous determination of 10 dyes in adulterated Phellodendron
|
|
Lim Su-Ji
Yun Choong-In
Ko Kyung-Yuk
Kim Young-Jun
|
|
|
Abstract
|
|
|
|
As global interest in herbal medicines has increased, the adulteration of herbal medicines has become a critical safety issue. Adulteration with dyes to improve the appearance of low-quality products is of particular concern. This study aimed to develop a high-performance liquid chromatography (HPLC) method to detect dyes added as adulterants to Phellodendron. Samples were analyzed on a C18 column using 50 mM ammonium acetate and acetonitrile as the mobile phase. All calibration curves showed good linearity (r2 ≥0.9999) over the five-point concentration range (1-50 mg/kg). Limit of detection ranged from 0.04-0.35 mg/kg, and limit of quantification ranged from 0.11-1.07 mg/kg. The repeatability and reproducibility for these measurements were 94.2-103.3% and 96.6-103.8% for accuracy and 0.14-2.28 RSD (%) and 0.80-2.37 RSD (%) for precision. Moreover, the measurement uncertainty of the low, medium, and high concentrations for 10 dyes was considered. Thus, this HPLC method is suitable for detecting color adulteration of Phellodendron.
|
|
|
KEYWORD
|
|
|
Adulteration , Herbal medicine , Measurement uncertainty , Synthetic dye , Validation
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
|